Timothy James Mott, 34, of Columbia Cross Roads, was charged after the crash early Saturday morning, the commission said.
A message seeking comment from Mott's lawyer, Patrick Beirne, was not immediately returned.
The Game Commission said a state police aircraft reported someone spotlighting wildlife after 2 a.m. in Bradford County, and its officers were able to locate the vehicle on the ground. State law prohibits recreational spotlighting between 11 p.m. and sunrise.
The agency said that after a chase Mott's vehicle struck a Game Commission vehicle.
Mott and wildlife conservation officers Jeffrey Oleniacz and John DeMille required hospital treatment.
Authorities said Mott's vehicle contained three loaded rifles and a spotlight.
Mott was charged with aggravated assault, fleeing from police, having a loaded gun in a vehicle, unlawful use of lights and traffic offenses. A preliminary hearing was scheduled for next week.