The World Anti-Doping Agency announced Tuesday it is withdrawing the lab's accreditation, less than 10 months before it is due to analyze players' samples for FIFA during the World Cup in Brazil.
"The laboratory—which is currently suspended—will no longer be authorized to carry out the testing of doping control samples on behalf of WADA or any testing authority," the anti-doping watchdog said in a statement.
Managers at the laboratory can file an appeal at the Court of Arbitration for Sport before the revocation takes effect on Sept. 25.
"It may also choose to reapply for accreditation, and can seek 'fast track' process from the executive committee," WADA said.
WADA suspended the Rio center on Aug. 8 from testing any anti-doping samples, though it has not specified how the lab failed.
"WADA may revoke a laboratory's accreditation for repeated failures to comply with the ISL (International Standard for Laboratories) and related technical documents," it said.
It is the second public embarrassment for Brazil's only WADA-accredited center during preparations to host the two biggest events in world sports.
In 2012, the Rio lab was barred from performing isotope ratio mass spectrometry tests, typically used to determine if steroids and hormones were produced in the body or outside it, for nine months after falsely accusing a Brazilian beach volleyball player of doping with testosterone.
Then, the International Volleyball Federation asked WADA to investigate a "regrettable incident."