The one day walkout Wednesday largely involves workers in the organization's London office.
The workers say that Amnesty has changed the rules on redundancy ahead of layoffs amid a planned reorganization of the human rights organization.
Amnesty says the organization respects the right of the workers to strike, and noted that only one-third of the workforce chose to do so.
The group says in a statement that it must change the way it operates to be more effective. That includes moving away from a centralized London base of 500 people to 10 regional hubs closer to where human rights violations occur.