Spokesman Michael DiMauro said the employees were given notice earlier this week.
He said some workers volunteered to be laid off, while others were "non-voluntary." He wasn't sure of the ratio.
Restructuring plans for the Springettsbury Township plant call for reducing the workforce from about 1,950 to 1,000, with between 700 and 800 full-time employees.
Layoffs will continue for the next 12 months, he said.
He couldn't immediately provide the number of layoffs that lie ahead, as attrition will affect the number, he said.
Earlier this week, the company posted a drop in profit for the first quarter of 2010. The $33.3 million, or 14 cents per share, in profit is a 36-cent per share drop from the $117.3 million posted during the same period of 2009.
Worldwide retail sales of new motorcycles fell 18.2 percent, which was an improvement in the rate of decline when compared to the prior three quarters.
Sales in the U.S. fell by more than 24 percent.
However, the company is on target with its guidance, shipping 53,674 bikes in the first quarter. It reiterated an earlier guidance that forecast it to ship between 201,000 and 212,000 motorcycles for the year, a decrease of between 5 and 10 percent from last year.
It plans to ship between 55,000 and 60,000 motorcycles in the second quarter.