The festival announced Tuesday that the screening will take place April 25 during the 16th annual Ebertfest at the Virginia Theatre in Champaign, Ill.
Ebert gave "Do The Right Thing" four stars when it debuted in 1989.
The festival previously announced that director Oliver Stone would attend a 25th-anniversary screening of his film "Born on the Fourth of July." Comedian Patton Oswalt will attend a screening of his 2011 movie "Young Adult."
Ebert died last April at age 70. The year's Ebertfest is April 23-27. The full lineup will be announced soon.
Ebert's alma mater, the University of Illinois, is based in Champaign and neighboring Urbana.